|Western Blot (WB)||1:1000|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Rat, Xenopus|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr15 of rat CDK1 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This antibody is predicted to react with mouse and zebrafish based on 100% sequence homology.
This antibody is specific for the ~38 kDa cdc2 protein phosphorylated at Tyr15 in Western blots of SK-N-MC total cell extracts. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle (Maller, 1991). The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2 (Nakamizo et al., 2002). Cdc2 expression in brain has been linked to both neurogenesis and apoptosis (Konishi and Bonni, 2003; Dranovsky et al., 2001; Okano et al., 1996).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CDK1; CDK1-A; cell cycle controller CDC2; Cell division control protein 2 homolog; Cell division control protein 2 homolog 1; Cell division control protein 2-A; cell division cycle 2 homolog A; cell division cycle 2 like; Cell division cycle 2 protein; cell division cycle 2, G1 to S and G2 to M; Cell division cycle control protein 2; Cell division protein kinase 1; Cell division protein kinase 1-A; Cyclin Dependent Kinase 1; Cyclin-dependent kinase 1; Cyclin-dependent kinase 1-A; p34 Protein Kinase; p34 protein kinase 1
Gene Aliases: cdc-2; CDC2; cdc2-a; CDC28A; Cdc2a; cdc2x1.1; CDK1; cdk1-a; CDKN1; P34CDC2; PSTAIR; xcdc2
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.