Keep as concentrated solution.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Japanese Medaka (100%), Drosophila (100%), Xenopus laevis (100%), Pig (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).
Positive Control: HeLa (untreated), HeLa (2mM double thymidine treatment and recovery for 3.5hr), HeLa (2mM double thymidine treatment and recovery for 12hr), HeLa (100 ng/mL Nocodazole treatment for 24hr), Rat-2 (untreated), Rat-2 (100 ng/mL Nocodazole treatment for 24.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
CDK1 (CDC2, P34CDC2) is a member of the Ser/Thr protein kinase family. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDK1 also play important regulatory roles in cell cycle control. Destruction of cyclin B during metaphase results in inactivation of CDK1, allowing mitotic exit and cell division. CDK1 also contributes to the control of DNA replication and can be inhibited by several transcriptional targets of p53, such as p21WAF. The ability of CDK1 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2. Research studies have shown that expression of CDK1 in the brain has been linked to both neurogenesis and apoptosis. In vertebrates, multiple cyclins like CDK1 have been identified, including a total of eight such regulatory proteins in mammals. Alternatively spliced transcript variants encoding different isoforms of CDK1 have been found.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ABL1; CDK1; cell cycle controller CDC2; Cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M; Cell division protein kinase 1; Cyclin-dependent kinase 1; DKFZp686L20222; MGC111195; MGC53829; OTTHUMP00000019660; P34 CDK1; P34 MGC111195; p34 protein kinase
Gene Aliases: CDC2; CDC28A; CDK1; CDKN1; P34CDC2
UniProt ID: (Human) P06493
Entrez Gene ID: (Human) 983