|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human C/EBP-beta around the phosphorylation site of Threonine 235|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous levels of human LAP only when phosphorylated at Thr235, mouse and rat LAP only when phosphorylated at Thr188, and LIP only when phosphorylated at Thr37 (molecular weight of 19, 36 and 38 kDa).
Purity is >95% by SDS-PAGE.
C/EBP beta is a member of the C/EBP transcription factor family. The C/EBP beta gene encodes several isoforms, which have truncated transcription activation domains by the alternative translational initiation at multipe AUG start sites. Initiation of translation at the in-frame AUGs forms four C/EBP beta isoforms. C/EBP beta is also known as interleukin 6-dependent DNA-binding protein (IL6DBP), liver activator protein(LAP) or liver-enriched transcriptional activator protein transcription factor 5 (TCF5). C/EBP beta contributes to the regulation of the acute phase response in hepatocytes. Stat3 has an important function in IL-6-mediated transcription of the C/EBP beta gene that has direct implication for acute phase response in liver cells.The 35 kDa C/EBP beta form requires phosphorylation for its DNA binding ability, and increased binding of C/EBP beta isoforms during acute-phase reaction occurs through its upregulation and structural modification.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.