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Phospho-CENP-A (Ser18) Polyclonal Antibody

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Phospho-CENP-A (Ser18) Antibody in Immunocytochemistry (ICC/IF) — CENP-A phospho Ser18 antibody (pAb) tested by immunofluorescence. Detection of CENP-A phospho Ser18 by immunofluorescence. HeLa cells were stained with CENP-A phospho Ser18 antibody at a 1:100 dilution. Green: CENP-A phospho Ser18 antibody (pAb). Blue: DAPI.

Phospho-CENP-A (Ser18) Antibody in Western Blot (WB) — CENP-A phospho Ser18 antibody (pAb) tested by Western blot. Top: Recombinant CENP-A (20 ng) was probed with CENP-A phospho Ser18 antibody (1:100 dilution) (A) or with anti-centromere antibody (ACA) (B). CENP-A phospho Ser18 antibody does not detect recombinant CENP-A. Bottom: HeLa nuclear extract (20 µg) was probed with CENP-A phospho Ser18 antibody (1:250 dilution). Lane 1: CENP-A phospho-Ser18 antibody. Lane 2: anti-centromere antibody (ACA).

Phospho-CENP-A (Ser18) Antibody in Dot blot (DB) — CENP-A phospho Ser18 antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of CENP-A phospho Ser18 antibody. Peptides corresponding to the immunogen and related proteins were spotted onto PVDF and probed with the antibody at 1:750. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: CENP-A phospho Ser 16 and phospho Ser18. Lane 2: CENP-A unmodified. Lane 3: CENP-A phospho Ser 16. Lane 3: CENP-A phospho Ser 16.

Product Details

61483

Applications	Tested Dilution	Publications
Western Blot (WB)	1:100-1:500	-
Immunocytochemistry (ICC/IF)	1:100	View 1 publication 1 publication
Dot blot (DB)	Assay-dependent	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	This antibody was raised against a synthetic peptide containing phosphorylated serine 16 and 18 of human CENP-A. View immunogen
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.5, with 30% glycerol
Contains	0.035% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2793655

Target Information

Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Both tails and the centromere targeting domain of CENP-A are required for centromere establishment.

The Journal of cell biology

Logsdon GA,Barrey EJ,Bassett EA,DeNizio JE,Guo LY,Panchenko T,Dawicki-McKenna JM,Heun P,Black BE

Research topic: Cell Division

Mon Mar 02 00:00:00 EST 2015

Bioinformatics

Protein Aliases: CENP A; CENP-A; Centromere autoantigen A; Centromere protein A; centromere protein A, 17kDa; centromere-specific histone; Histone H3-like centromeric protein A

Gene Aliases: CenH3; CENP-A; CENPA

UniProt ID: (Human) P49450

Entrez Gene ID: (Human) 1058

Molecular Function: chromatin/chromatin-binding, or -regulatory protein histone