|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of threonine383 (M-R-T(p)-L-C) derived from Human Chk1.|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
A suggested positive control for Western blot is COS7 cells; suggested positive control for ICC/IF is Hela cells.
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both functionas essential components in the G2 DNA damage checkpoint by phophorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cds1; Cds1 homolog; Checkpoint kinase 2; checkpoint-like protein CHK2; CHEK2; CHK2; CHK2 checkpoint homolog; EC 188.8.131.52; Hucds1; kinase Chk2; protein kinase Chk2; RAD53; Rad53 homolog; Serine/threonine-protein kinase Chk2
Gene Aliases: CDS1; CHEK2; CHK2; hCds1; HUCDS1; LFS2; PP1425; RAD53
Molecular Function: non-receptor serine/threonine protein kinase
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