Invitrogen

Phospho-CK1 alpha (Thr321) Polyclonal Antibody

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Phospho-CK1 alpha (Thr321) Antibody in Western Blot (WB) — Western blot analysis of Phospho-CK1 alpha (Thr321) in Lane 1: HEK293T cell lysate treated with UV (4h), Lane 2: mouse liver tissue lysate, Lane 3: H9C2 cell lysate treated with UV (4h). Samples were incubated with Phospho-CK1 alpha (Thr321) polyclonal antibody (Product # PA5-36790) at a dilution of 1:500.

Product Details

PA5-36790

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic phosphopeptide derived from human Casein Kinase I alpha around the phosphorylation site of Threonine 321 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2553736

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 42 kDa.

Purity is >95% by SDS-PAGE.

Target Information

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Methazolamide Attenuates the Development of Diabetic Cardiomyopathy by Promoting β-Catenin Degradation in Type 1 Diabetic Mice.

Diabetes

Chen X,Li Y,Yuan X,Yuan W,Li C,Zeng Y,Lian Y,Qiu X,Qin Y,Zhang G,Liu X,Luo C,Luo JD,Hou N

PA5-36790 was used in Western Blot to help inform novel therapeutic strategy to prevent heart failure in patients with diabetes.

Fri Apr 01 00:00:00 EDT 2022

A Brain Signaling Framework for Stress-Induced Depression and Ketamine Treatment Elucidated by Phosphoproteomics.

Frontiers in cellular neuroscience

Xiao Y,Luo H,Yang WZ,Zeng Y,Shen Y,Ni X,Shi Z,Zhong J,Liang Z,Fu X,Tu H,Sun W,Shen WL,Hu J,Yang J

PA5-36790 was used in Western Blotting to investigate phosphoprotein changes induced by chronic unpredictable mild stress (CUMS) and ketamine treatment in two known mood control centers, the medial prefrontal cortex (mPFC) and the nucleus accumbens (NAc) in mice.

Mon Sep 28 00:00:00 EDT 2020

A Brain Signaling Framework for Stress-Induced Depression and Ketamine Treatment Elucidated by Phosphoproteomics.

Frontiers in cellular neuroscience

Xiao Y,Luo H,Yang WZ,Zeng Y,Shen Y,Ni X,Shi Z,Zhong J,Liang Z,Fu X,Tu H,Sun W,Shen WL,Hu J,Yang J

Mon Sep 28 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: casein kinase 1, alpha 1; Casein kinase I isoform alpha; casein kinase I-alpha; CK I alpha; CK-I alpha; CK1; CKI alpha; CKI-alpha; clock regulator kinase; CSNK1A1/CK1; down-regulated in lung cancer; epididymis secretory sperm binding protein Li 77p; OTTHUMP00000224000; OTTHUMP00000224001; OTTHUMP00000224003; YIL035C

Gene Aliases: 2610208K14Rik; 4632404G05Rik; 5430427P18Rik; CK1; CK1a; CKIa; Csnk1a; CSNK1A1; HEL-S-77p; HLCDGP1; PRO2975

UniProt ID: (Human) P48729, (Rat) P97633

Entrez Gene ID: (Human) 1452, (Rat) 113927, (Mouse) 93687

Molecular Function: non-receptor serine/threonine protein kinase