Invitrogen

Phospho-CK1 alpha (Thr321) Polyclonal Antibody

Product Image Gallery

Phospho-CK1 alpha (Thr321) Antibody in Western Blot (WB) — Western blot analysis of Phospho-CK1 alpha (Thr321) in Lane 1: HEK293T cell lysate treated with UV (4h), Lane 2: mouse liver tissue lysate, Lane 3: H9C2 cell lysate treated with UV (4h). Samples were incubated with Phospho-CK1 alpha (Thr321) polyclonal antibody (Product # PA5-36790) at a dilution of 1:500.

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Western Blot (WB)

1:500-1:1000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide derived from human Casein Kinase I alpha around the phosphorylation site of Threonine 321

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2553736

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 42 kDa.

Purity is >95% by SDS-PAGE.

Target Information

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: casein kinase 1, alpha 1; Casein kinase I isoform alpha; casein kinase I-alpha; CK I alpha; CK-I alpha; CK1; CKI alpha; CKI-alpha; clock regulator kinase; CSNK1A1/CK1; down-regulated in lung cancer; epididymis secretory sperm binding protein Li 77p; OTTHUMP00000224000; OTTHUMP00000224001; OTTHUMP00000224003; YIL035C

Gene Aliases: 2610208K14Rik; 4632404G05Rik; 5430427P18Rik; CK1; CK1a; CKIa; Csnk1a; CSNK1A1; HEL-S-77p; HLCDGP1; PRO2975

UniProt ID: (Human) P48729, (Rat) P97633, (Mouse) Q8BK63

Entrez Gene ID: (Human) 1452, (Rat) 113927, (Mouse) 93687

Molecular Function: kinase non-receptor serine/threonine protein kinase protein kinase transferase