|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A phospho-peptide spanning the Ser-121 phosphorylation site of human CREB.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris with 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||0.5 µg|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: antigen standard for CREB1 (transient overexpression lysate).
Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. CREB is also regulated by DNA-damaging stimuli and is directly phosphorylated on Ser-121 by the ataxia-telangiectasia-mutated protein kinase, ATM in response to ionizing radiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.