Western blot of a forskolin stimulated rat hippocampal lysate showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 (Lane 1). The right lane is indicative that the specific immunolabeling of the CREB protein is completely eliminated by treatment with lambda phosphatase (Lane 2) (1200 units for 30 min).
|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser133 of rat CREB1 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine, chicken, human, mouse, non-human primate, rat, sheep, Xenopus and zebrafish based on 100% sequence homology.
This antibody is specific for the ~45 kDa CREB phosphorylated at Ser133 in Western blots of rat brain lysates. Immunolabeling can be blocked by lambda-phosphatase treatment.
It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenber , 1999; Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser133), and phosphorylation of Ser133 are required for CREB-mediated transcription (Johannessen et al., 2004; Kornhauser et al., 2002).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.