|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to a portion of human CaM Kinase II alpha containing threonine 286.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26727 detects CaMKII alpha (phospho T286) from human samples. PA1-26727 is expected to cross react with mouse (100% conserved), and rat (100% conserved) due to sequence homology.
PA1-26727 has been successfully used in Western blot procedures.
The PA1-26727 immunogen is a synthetic phosphopeptide corresponding to a portion of human CaM Kinase II alpha containing threonine 286.
Store at 4°C for short term storage. For long term storage store at -20°C or below. Avoid Freeze/Thaw Cycles.
Calcium/calmodulin-dependent protein kinase II alhpa (CaM Kinase II alhpa) is a 50 kDa member of CaM Kinase II family of serine-theonine kinase that transduce Ca2+ signals to several target proteins, including ion channels and transcription activators. CaM Kinase II is predominantly expressed in two isoforms in the brain: alpha and beta. CaM Kinase II playa an important role in neuronal plasticity and memory formation, and exerts both calcium-calmodulim-dependent and -independent activities. Autophosphorylation of CaM Kinase II alpha on threonine 286 allows the kinase to switch from calmodulin-dependent to a calmodulin-independent state, and is required for varios cellular functions including hippocampal long-term potential (LPT), special learning,, and hippocampus-dependent memory.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.