MA1-047 detects phosphorylated CaM kinase II from human, rat and mouse tissues/cells. This antibody has been shown to detect the phosphorylated alpha subunit CaM kinase II only. MA1-047 does not detect the non-phosphorylated alpha subunit CaM kinase II nor with the ~60 kDa beta subunit in either phosphorylation state.
MA1-047 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, immunocytochemistry, immunohistochemistry, and ELISA procedures. By Western blot, this antibody detects an ~50 kDa band representing the alpha subunit of CaM kinase II only after phosphorylation. Immunofluorescence staining of CaM kinase II in rat/mouse hippocampal cells with MA1-047 densely labels cell somas and neuropil areas, while nuclei are only lightly stained.
The MA1-047 immunizing peptide is a synthetic peptide corresponding to residues M(281) H R Q E T(p) V D C L K K F N(294) of rat CaM Kinase II protein with a phosphorylated threonine at position 286.
CaM kinase II, in a calcium and calmodulin dependent manner, phosphorylates many different brain substrates including synapsin I, tryptophan hydroxylase, tyrosine hydroxylase and nitric oxide synthase thereby performing regulatory functions associated with increases in intracellular free calcium. CaM kinase II is particularly abundant in the hippocampus and forebrain where it comprises ~1% of total protein. Within the neuron, CaM kinase II is a major component of the postsynaptic density fraction accounting for as much as 30% of total protein in the post synaptic density (PSD) region.
Autophosphorylation of threonine-286 occurs after calcium/calmodulin activation and can enable CaM kinase II to remain active independent of free calcium. Autophosphorylation of CaM kinase II in hippocampal neurons, which results in relatively high levels of kinase activity even at basal calcium concentrations, may allow for both upward and downward regulation as opposed to simply on/off regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: alpha CaM kinase II; alpha-CaMKII; Ca2+/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase II alpha-B subunit; Calcium/calmodulin-dependent protein kinase type 2; calcium/calmodulin-dependent protein kinase type II alpha chain; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II alpha subunit; CaM kinase II subunit alpha; CaM-kinase II alpha chain; CaMK II; CaMK-II alpha subunit; CaMK-II subunit alpha; CaMKIINalpha; Phospho-CaMKI
Gene Aliases: CAMK2A; CAMKA; CaMKII; KIAA0968; mKIAA0968; PK2CDD; PKCCD; R74975
Molecular Function: non-receptor serine/threonine protein kinase