Invitrogen

Phospho-CaMKII alpha/beta (Thr286) Polyclonal Antibody

2 References
View all (5) CaMKII alpha/beta antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA1-4614

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
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Immunohistochemistry (IHC)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rabbit, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 of rat CAMK2A/B conjugated to KLH
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2259386

Product Specific Information

This antibody is predicted to react with human, mouse and Xenopus based on 100% sequence homology.

This antibody is specific for the ~50 kDa alpha-CaM Kinase II subunit and the ~60 kDa beta-CaM Kinase II subunit phosphorylated at Thr286 in Western blots of rat brain lysates. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

Target Information

Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular response to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduces LTP and learning (Elgersma et al., 2002).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alpha CaM kinase II; alpha-CaMKII; Ca++/calmodulin-dependent protein kinase II beta subunit; Ca2+/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II beta; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase II beta; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase type II beta chain; Calcium/calmodulin-dependent protein kinase type II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II alpha subunit; CaM kinase II beta subunit; CaM kinase II subunit alpha; CaM kinase II subunit beta; CaM-kinase II alpha chain; CaM-kinase II beta chain; CaMK II; CaMK-II alpha subunit; caMK-II subunit alpha; caMK-II subunit beta; CaMKIINalpha; proline rich calmodulin-dependent protein kinase

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Gene Aliases: CAM2; CAMK2; CAMK2A; CAMK2B; Camk2d; CAMKA; CAMKB; CaMKII; Ck2b; KIAA0968; mKIAA0968; PK2CDD; PKCCD; R74975

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UniProt ID: (Human) Q9UQM7, (Human) Q13554, (Mouse) P11798, (Rat) P11275, (Rat) P08413, (Mouse) P28652

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Entrez Gene ID: (Human) 815, (Rabbit) 100341507, (Human) 816, (Rabbit) 100342451, (Mouse) 12322, (Rat) 25400, (Rat) 24245, (Mouse) 12323

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Function(s)
protein serine/threonine kinase activity calmodulin-dependent protein kinase activity Ras guanyl-nucleotide exchange factor activity protein binding calmodulin binding ATP binding kinase activity glutamate receptor binding protein homodimerization activity actin binding protein kinase binding phospholipase binding nucleotide binding protein kinase activity transferase activity transferase activity, transferring phosphorus-containing groups GTPase activating protein binding non-receptor serine/threonine protein kinase
Process(es)
G1/S transition of mitotic cell cycle MAPK cascade response to ischemia protein phosphorylation calcium ion transport Wnt signaling pathway, calcium modulating pathway positive regulation of cardiac muscle cell apoptotic process peptidyl-serine phosphorylation angiotensin-activated signaling pathway positive regulation of GTPase activity protein autophosphorylation regulation of neurotransmitter secretion regulation of neuronal synaptic plasticity positive regulation of NF-kappaB transcription factor activity positive regulation of calcium ion transport interferon-gamma-mediated signaling pathway regulation of cellular response to heat regulation of mitochondrial membrane permeability involved in apoptotic process signal transduction positive regulation of neuron projection development regulation of skeletal muscle adaptation positive regulation of phospholipase A2 activity regulation of long-term neuronal synaptic plasticity cell projection morphogenesis regulation of synapse structural plasticity regulation of calcium ion transport regulation of dendritic spine development positive regulation of dendritic spine morphogenesis positive regulation of synapse maturation phosphorylation ionotropic glutamate receptor signaling pathway inhibitory G-protein coupled receptor phosphorylation regulation of synaptic transmission, cholinergic response to cadmium ion neuromuscular process controlling balance long-term synaptic potentiation activation of meiosis involved in egg activation positive regulation of apoptotic signaling pathway nervous system development cell differentiation
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