Western blot analysis of Phospho-Cardiac-Troponin I pSer43 in mouse heart lysate using a Phospho-Cardiac-Troponin I pSer43 polyclonal antibody (Product # PA5-35412). Results show a band at ~25kDa. Phosphospecificity is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that the lysate was incubated in lambda-Ptase (1400 units for 30 min). The immunolabeling is greatly decreased by treatment with lambda-Ptase.
|Tested species reactivity||Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser43 of mouse TNNI3 conjugated to KLH|
|Storage buffer||0.1M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 0.1mg/ml BSA|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine, human, non-human primate, sheep, Xenopus and zebrafish based on 100% sequence homology.
This antibody contains enough material to conduct 10 mini-Western blots.
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.