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Western blot analysis of Phospho-Caveolin 2 pSer36 using 30 mg of lysates from LNCaP cells expressing Caveolin-2 incubated without (lane 1) or with calf intestinal phosphatase (CIP; lane 2). Samples were resolved on 12% SDS-PAGE, transferred onto nitrocellulose membrane and probed with a total Caveolin-2 non-phospho serine 23 antibody (Product # PA1-25624; top panel), phospho-serine 23 (Product # PA1-25626; middle panel), and phospho-serine 36 (Product # PA1-25626; bottom panel).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 32-45 of human caveolin 2, phosphorylated on serine 36.|
|Purification||Antigen affinity chromatography|
|Storage buffer||1% BSA, 50% glycerol|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-25626 detects Caveolin 2 (phospho S36) from human samples.
PA1-25626 has been successfully used in Western blot procedures.
The PA1-25626 immunogen is a synthetic peptide corresponding to residues 32-45 of human caveolin 2, phosphorylated on serine 36.
The protein encoded by this gene is a major component of the inner surface of caveolae, small invaginations of the plasma membrane, and is involved in essential cellular functions, including signal transduction, lipid metabolism, cellular growth control and apoptosis. This protein may function as a tumor suppressor. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Two transcript variants encoding distinct isoforms have been identified for this gene. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by one transcript.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CAV; caveolae protein, 20-kD; caveolin 2 isoform a and b; MGC12294