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Phospho-Chk2 (Thr68) Antibody (PA5-104715) in ICC/IF
Immunofluorescent analysis of Phospho-Chk2 (Thr68) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-Chk2 (Thr68) polyclonal antibody (Product # PA5-104715) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (r... View More
Product Details
PA5-104715
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CHEK2(Accession O96017), corresponding to amino acid residues around phosphorylated Thr68.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2816188
Product Specific Information
Antibody detects endogenous levels of Chk2 only when phosphorylated at Threonine 68.
Target Information
CHEK2 (CHK2) is a serine/threonine kinase and a component of the DNA damage checkpoint pathway. A mutation in CHK2 has been linked to cancer. CHK2 is activated by DNA damage and phosphorylates several modulators of cell cycle control including tumor suppressor proteins. In addition, Chk2 can phosphorylate BRCA1, allowing BRCA1 to restore survival after DNA damage. Chk2 is a putative tumor suppressor protein, with mutations in to the Chk2 gene linked to Li-Fraumeni syndrome, a familiar cancer usually associated with inherited mutations in TP53. Also, mutations in CHK2 are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cds1 homolog; Checkpoint kinase 2; Checkpoint like protein CHK2; checkpoint-like protein CHK2; Chk2 (phospho T68); Chk2 (pThr68); CHK2 checkpoint homolog; CHK2 checkpoint homolog (S. pombe); Chk2 phospho; Chk2 phospho T68; del9; EC 2.7.11.1; HuCds 1; Hucds1; kinase Chk2; OTTHUMP00000199044; OTTHUMP00000199045; OTTHUMP00000199064; OTTHUMP00000199115; OTTHUMP00000199116; phospho Chk2 (T68); phospho Thr68 Chk2; protein kinase Chk2; Rad53 homolog; RP11-436C9.1; Serine/threonine protein kinase Chk2; Serine/threonine-protein kinase Chk2
Gene Aliases: CDS1; CHEK2; CHK2; hCds1; HUCDS1; LFS2; PP1425; RAD53
UniProt ID: (Human) O96017, (Mouse) Q9Z265
Entrez Gene ID: (Human) 11200, (Mouse) 50883, (Rat) 114212
protein serine/threonine kinase activity
protein binding
ATP binding
protein kinase binding
ubiquitin protein ligase binding
identical protein binding
protein homodimerization activity
metal ion binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
DNA damage checkpoint
G2/M transition of mitotic cell cycle
replicative cell aging
positive regulation of protein phosphorylation
double-strand break repair
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
cellular response to DNA damage stimulus
DNA damage induced protein phosphorylation
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
intrinsic apoptotic signaling pathway in response to DNA damage
response to gamma radiation
peptidyl-serine phosphorylation
cellular response to drug
regulation of protein catabolic process
signal transduction in response to DNA damage
cellular protein catabolic process
positive regulation of transcription, DNA-templated
protein autophosphorylation
protein stabilization
cell division
negative regulation of cell cycle arrest
signal transduction involved in intra-S DNA damage checkpoint
mitotic spindle assembly
replicative senescence
regulation of signal transduction by p53 class mediator
response to doxorubicin
cellular response to bisphenol A
negative regulation of DNA damage checkpoint
positive regulation of anoikis
DNA repair
apoptotic process
cell cycle
mitotic nuclear division
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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