|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to aa 64-73 of human Chk2.|
|Contains||0.01% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Western Blot (WB)||1/200-1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Predicted to react with chimpanzee, mouse and rat based on sequence homology.
For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM sodium orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background. In Western blot, this antibody detects a band at ~61 kDa.
Checkpoint kinase 2 (Chk2) is a serine/threonine-protein kinase which functions as a cell cycle checkpoint regulator. ATM and ATR kinases are activated in response to ionizing and ultraviolet radition, and phosphorylate Thr68 and other sites in the amino-terminal region of Chk2 dimerisation and activation. Activated Chk2 phosphorylates and inhibits Cdc25C phosphates, preventing the entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, Chk2 can phosphorylate BRCA1, allowing BRCA1 to restore survival after DNA damage. Chk2 is a putative tumor suppressor protein, with mutations in to the Chk2 gene linked to Li-Fraumeni syndrome, a familiar cancer usually associated with inherited mutations in TP53.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.