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Invitrogen
Phospho-Cofilin (Ser3) Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
Phospho-Cofilin (Ser3) Antibody (44-1072G) in WB
Western blot analysis was performed on whole cell extracts (20 µg lysate) of HeLa (Lane 1), HeLa treated for overnight with 150 uM of H2O2 (Lane 2), HeLa treated for overnight with 3 uM of Staurosporine (lane 3), COS-7 (lane 4), NIH/3T3 (lane 5), MCF7 (lane 6), Rat Skeletal Muscle (lane 7), A-431 (lane 8), A-431 treated for overnight with 150 uM of H2O2 (lane 9), Jurkat (lane 10) and Jurkat treated for over... View More
Product Details
44-1072G
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human cofilin that contains serine 3. The sequence is conserved in mouse and rat.
Conjugate
Unconjugated
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
RRID
AB_2533565
Target Information
Cofilin binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 18 kDa phosphoprotein; CFL-1; COF1; cofilin 1 (non-muscle); Cofilin, non-muscle isoform; Cofilin-1; Cofilin1; epididymis secretory protein Li 15; p18
Gene Aliases: AA959946; CFL; CFL1; Cof; cofilin; HEL-S-15; n-cofilin
UniProt ID: (Human) P23528, (Rat) P45592, (Mouse) P18760
Entrez Gene ID: (Human) 1072, (Dog) 476022, (Rat) 29271, (Mouse) 12631
Molecular Function:
non-motor actin binding protein
cytoskeleton organization
Rho protein signal transduction
response to virus
regulation of cell morphogenesis
actin cytoskeleton organization
actin filament depolymerization
negative regulation of apoptotic process
positive regulation by host of viral process
regulation of dendritic spine morphogenesis
mitotic cytokinesis
neural crest cell migration
neural fold formation
protein phosphorylation
protein import into nucleus
actin filament organization
establishment of cell polarity
cell projection organization
positive regulation of actin filament depolymerization
response to amino acid
negative regulation of cell size
movement of cell or subcellular component
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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