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          • Primary Antibodies ›
          • Cofilin Antibodies

          Invitrogen

          Phospho-Cofilin (Ser3) Polyclonal Antibody

          Advanced Verification

          This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

          1 Published Figure
          2 References
          View all (36) Cofilin antibodies
          Datasheet
          Protocols
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          Tech Support

          Cite Phospho-Cofilin (Ser3) Polyclonal Antibody

          Datasheet
          Protocols
          Questions & Answers
          Tech Support
          • Antibody Testing Data (3)
          • Published Figures (1)
          • Advanced Verification (1)
          Phospho-Cofilin (Ser3) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          Phospho-Cofilin (Ser3) Antibody (44-1072G) in WB

          Western blot analysis was performed on whole cell extracts (20 µg lysate) of HeLa (Lane 1), HeLa treated for overnight with 150 uM of H2O2 (Lane 2), HeLa treated for overnight with 3 uM of Staurosporine (lane 3), COS-7 (lane 4), NIH/3T3 (lane 5), MCF7 (lane 6), Rat Skeletal Muscle (lane 7), A-431 (lane 8), A-431 treated for overnight with 150 uM of H2O2 (lane 9), Jurkat (lane 10) and Jurkat treated for over... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          View Product

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          Product Details

          44-1072G

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          -

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:250
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Dog, Human, Mouse, Rat

          Published species

          Not Applicable

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human cofilin that contains serine 3. The sequence is conserved in mouse and rat.
          View immunogen

          Conjugate

          Unconjugated

          Form

          Liquid

          Purification

          Antigen affinity chromatography

          Storage buffer

          Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C

          RRID

          AB_2533565

          Target Information

          Cofilin binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: 18 kDa phosphoprotein; CFL-1; COF1; cofilin 1 (non-muscle); Cofilin, non-muscle isoform; Cofilin-1; Cofilin1; epididymis secretory protein Li 15; p18

          View more View less

          Gene Aliases: AA959946; CFL; CFL1; Cof; cofilin; HEL-S-15; n-cofilin

          View more View less

          UniProt ID: (Human) P23528, (Rat) P45592, (Mouse) P18760

          View more View less

          Entrez Gene ID: (Human) 1072, (Dog) 476022, (Rat) 29271, (Mouse) 12631

          View more View less

          Molecular Function: non-motor actin binding protein

          Function(s)
          cytoskeleton organization Rho protein signal transduction response to virus regulation of cell morphogenesis actin cytoskeleton organization actin filament depolymerization negative regulation of apoptotic process positive regulation by host of viral process regulation of dendritic spine morphogenesis mitotic cytokinesis neural crest cell migration neural fold formation protein phosphorylation protein import into nucleus actin filament organization establishment of cell polarity cell projection organization positive regulation of actin filament depolymerization response to amino acid negative regulation of cell size movement of cell or subcellular component
          Process(es)
          protein binding actin filament binding actin binding
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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