Immunohistochemistry analysis of Phospho-Connexin 43 [pS368] showing staining in the membrane and cytoplasm of paraffin-embedded mouse heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Phospho-Connexin 43 [pS368] polyclonal antibody (483000) diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Bovine, Dog, Chicken, Guinea pig, Human, Mouse, Rat, Zebrafish|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Phosphopeptide corresponding to amino acid residues surrounding the phosphoserine 368 of rat connexin 43|
|Storage buffer||0.01M HEPES, pH 7.5, with 50% glycerol, 100µg/ml BSA, 0.15M NaCl|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
This gene encodes a member of the gap junction protein family. The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
iPS programmed without c-MYC yield proficient cardiogenesis for functional heart chimerism.
48-3000 was used in immunocytochemistry to discuss methods to generate induced pluripotent stem cells
|Martinez-Fernandez A,Nelson TJ,Yamada S,Reyes S,Alekseev AE,Perez-Terzic C,Ikeda Y,Terzic A||Circulation research (105:648)||2009|