Western blot of Phospho-DAP Kinase 1 pSer308 in whole extract of HEK-293 cells overexpressing human DAP Kinase WT (Lane 1) and HEK-293 cells overexpressing human DAP-Kinase (mutant S308A). Samples were separated on SDS-PAGE and probed using a Phospho-DAP Kinase 1 pSer308 monoclonal antibody (Product # MA1-24738) at a dilution of 1 µg/mL and detected using a Goat anti-mouse IgG-Peroxidase and chemiluminescent substrate.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic phosphopeptide corresponding to residues 303-312 of human DAP Kinase 1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-24738 detects DAP Kinase 1 (phospho S308) from human samples.
MA1-24738 has been successfully used in immunoprecipitation procedures.
The MA1-24738 immunogen is a synthetic phosphopeptide corresponding to residues 303-312 of human DAP Kinase 1.
Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.