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Product Details
PA5-36844
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:1,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:200 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Synthetic phosphopeptide derived from human DARPP-32 around the phosphorylation site of Threonine 34 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.2, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2553773 |
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 32 kDa.
Purity is >95% by SDS-PAGE.
Target Information
DARPP-32 is a dopamine (DA) and AMP-regugated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons. The protein inhibits Protein Phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regugation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac®.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DARPP-32; dopamine and cAMP-regulated neuronal phosphoprotein 32; Dopamine- and cAMP-regulated neuronal phosphoprotein; dopamine- and cAMP-regulated phosphoprotein DARPP-32; FLJ20940; IPPD; neuronal phosphoprotein DARPP-32; OTTHUMP00000164275; OTTHUMP00000164276; PPR1B; protein pho; Protein phosphatase 1 regulatory subunit 1B
Gene Aliases: AU040756; DARPP-32; DARPP32; PPP1R1B
UniProt ID: (Human) Q9UD71, (Rat) Q6J4I0, (Mouse) Q60829
Entrez Gene ID: (Human) 84152, (Rat) 360616, (Mouse) 19049
Molecular Function:
phosphatase inhibitor
response to amphetamine
transcription, DNA-templated
signal transduction
negative regulation of female receptivity
visual learning
intracellular signal transduction
negative regulation of protein serine/threonine kinase activity
negative regulation of protein kinase activity
negative regulation of catalytic activity
protein kinase inhibitor activity
cAMP-dependent protein kinase inhibitor activity
protein phosphatase inhibitor activity
protein serine/threonine phosphatase inhibitor activity
protein binding
protein phosphatase type 1 regulator activity
D1 dopamine receptor binding
D2 dopamine receptor binding
D3 dopamine receptor binding
D4 dopamine receptor binding
D5 dopamine receptor binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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