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|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 of rat PPP1R1B conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine, chicken, human, mouse and non-human primate based on 100% sequence homology.
In Western blot, this antibody detects an ~32 kDa protein representing the Phospho-Thr75 DARPP-32 protein.
DARPP-32 is a dopamine (DA) and cAMP-regulated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein; dopamine- and cAMP-regulated phosphoprotein DARPP-32; neuronal phosphoprotein DARPP-32; Protein phosphatase 1 regulatory subunit 1B
Darpp-32; Darpp32; Ppp1r1b