Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 2
Phospho-DDR1 (Tyr513) Antibody (PA5-104588) in ICC/IF
Immunocytochemistry analysis of Phospho-DDR1 (Tyr513) in Heat-shock treated Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-DDR1 (Tyr513) polyclonal antibody (Product # PA5-104588) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H... View More
Product Details
PA5-104588
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human DDR1(Accession Q08345), corresponding to amino acid residues around phosphorylated Tyr513.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2816063
Product Specific Information
Antibody detects endogenous levels of DDR1 only when phosphorylated at Tyr513.
Target Information
DDR1 is a receptor tyrosine kinase activated by collagen and is reported to regulate adhesion, cell migration and other cellular processes. Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI). In situ studies and Northern-blot analysis showed that expression of this encoded protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, this protein is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain. This gene is located on chromosome 6p21.3 in proximity to several HLA class I genes. Alternative splicing of this gene results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CD167 antigen-like family member A; CD167a; Cell adhesion kinase; DAAP-278B20.1; DDR1d; discoidin domain receptor family, member 1; Discoidin receptor tyrosine kinase; Epithelial discoidin domain receptor 1; Epithelial discoidin domain-containing receptor 1; HGK2; Mammary carcinoma kinase 10; MCK-10; neuroepithelial tyrosine kinase; neurotrophic tyrosine kinase receptor type 4; neurotrophic tyrosine kinase, receptor, type 4; protein-tyrosine kinase 3; Protein-tyrosine kinase 3A; Protein-tyrosine kinase MPK-6; protein-tyrosine kinase PTK-3; Protein-tyrosine kinase RTK-6; PTK3A protein tyrosine kinase 3A; TRK E; Tyrosine kinase DDR; Tyrosine-protein kinase CAK
Gene Aliases: 6030432F18; AI323681; CAK; CD167; CD167a; DDR; DDR1; Drd1; EDDR1; HGK2; MCK10; Mpk6; NEP; NTRK4; PTK3; PTK3A; PTK3D; RTK6; TRKE
UniProt ID: (Human) Q08345, (Mouse) Q03146
Entrez Gene ID: (Human) 780, (Mouse) 12305, (Rat) 25678
transmembrane receptor protein tyrosine kinase activity
protein binding
collagen binding
ATP binding
protein tyrosine kinase collagen receptor activity
metal ion binding
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
molecular_function
transmembrane signal receptor
regulation of cell growth
regulation of cell-matrix adhesion
cell adhesion
embryo implantation
lactation
negative regulation of cell proliferation
regulation of extracellular matrix disassembly
smooth muscle cell migration
extracellular matrix organization
organ regeneration
collagen-activated tyrosine kinase receptor signaling pathway
peptidyl-tyrosine autophosphorylation
ear development
skin development
wound healing, spreading of cells
protein autophosphorylation
branching involved in mammary gland duct morphogenesis
mammary gland alveolus development
smooth muscle cell-matrix adhesion
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
female pregnancy
phosphorylation
peptidyl-tyrosine phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support