Western blot analysis of Phospho-DGCR8 pSer377 in mouse nuclei lysate using a Phospho-DGCR8 pSer377 polyclonal antibody (Product # PA5-35385). Results show a band at ~120kDa. The immunolabeling is blocked by the phosphopeptide used as the antigen (Phos-pep) but not by the corresponding dephosphopeptide (not shown).
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser377 of human DGCR8 conjugated to KLH|
|Storage buffer||0.1M HEPES, pH 7.5, with 0.15M NaCl, 0.1mg/ml BSA, 50% glycerol|
|Storage Conditions||-20°C or -80°C if preferred|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody contains enough material to conduct 10 mini-Western blots.
This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.