Western blot detection of Phospho-DNA PKcs pThr2609 in 20µg of: (Lane 1) Fus1 untreated (Lane 2) Fus1 IR (20Gy, 4h) (Lane 3) Fus1 DNAPK inhibitor + IR (Lane 4) MO59J (DNAPK-) untreated (Lane 5) MO59J IR (Lane 6) Fus1 untreated (Lane 7) Fus1 IR (20Gy, 4h) (Lane 8) Fus1 DNAPK inhibitor + IR (Lane 9) MO59J untreated (Lane 10) MO59J IR. Lanes 1-5 are nuclear extract whereas Lanes 6-10 are whole cell lysates. Samples were run on SDS-PAGE, transferred onto nitrocellulose, and probed using a Phospho-DNA PKcs pThr2609 polyclonal antibody (Product # PA1-29541) at a dilution of 1:1000 followed by detection using a 1:5000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature and Chemiluminescent detection at a 1 min exposure time. MO59J is a cell line that lacks DNA-PKcs. FUS1 is the matched cell line complemented with a chromosomal fragment containing the DNA-PKcs gene.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 2604-2614 of human DNA PKcs corresponding to phosphorylated at the pT2609 residue|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29541 detects DNA PKcs from human samples.
PA1-29541 has been successfully used in Western blot applications.
The PA1-29541 immunogen is: Synthetic peptide - amino acids 2604-2614 of human DNA PKcs - phosphorylated at the pT2609 residue
DNA-PK is a trimeric nuclear protein that contains the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) and the Ku heterodimer. These proteins are required for the repair of DNA double-strand breaks and V(D)J recombination. DNA-PKcs is a 460kDa
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.