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Phospho-DNAJC2 (Ser49) Antibody (PA5-105692) in ICC/IF
Immunocytochemistry analysis of Phospho-DNAJC2 (Ser49) in HepG2 cells (4h of LPS treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-DNAJC2 (Ser49) polyclonal antibody (Product # PA5-105692) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated go... View More
Product Details
PA5-105692
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human DNAJC2(Accession Q99543), corresponding to amino acid residues around phosphorylated Ser49.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2817120
Product Specific Information
Antibody detects endogenous levels of DNAJC2/MPP11 only when phosphorylated at Ser49.
Target Information
EIF4EBP1 is one member of a family of translation repressor proteins. It directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DnaJ (Hsp40) homolog, subfamily C, member 2; DnaJ homolog subfamily C member 2; Id associate 1; M-phase phosphoprotein 11; MIDA1; Mouse Id associate 1; zuotin related factor 1; zuotin related factor 2; Zuotin-related factor 1
Gene Aliases: AU020218; DNAJC2; MIDA1; MPHOSPH11; MPP11; ZRF1; Zrf2; ZUO1
UniProt ID: (Human) Q99543, (Mouse) P54103
Entrez Gene ID: (Human) 27000, (Mouse) 22791
DNA replication
transcription, DNA-templated
covalent chromatin modification
negative regulation of cell growth
positive regulation of ATPase activity
positive regulation of transcription, DNA-templated
'de novo' cotranslational protein folding
regulation of cellular response to heat
negative regulation of DNA biosynthetic process
regulation of transcription, DNA-templated
chromatin modification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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