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Invitrogen
Phospho-DNM1L (Ser616) Recombinant Rabbit Monoclonal Antibody (PSH06-77)
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FIGURE: 1 / 11
Phospho-DNM1L (Ser616) Antibody (MA5-56582) in ICC/IF
Immunofluorescence analysis of Phospho DNM1L was performed using 70% confluent log phase HEK-293 cells treated with nocodazole at 100 ng/mL for 17 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Phospho-DNM1L (Ser616) Recombinant Rabbit Monoclonal Ant... View More
Product Details
MA5-56582
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH06-77
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser616 of human DRP1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Product Specific Information
Positive Control: HeLa cell lysate, HeLa treated with 100 ng/mL Calyculin A for 30 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 100 ng/mL nocodazole for 18 hours cell lysate, HeLa, C2C12 cell lysate, PC-12 cell lysate, mouse brain tissue, Jurkat cell lysate, HEK-293 cell lysate, Human brain tissue lysate, Mouse brain tissue lysate, Mouse heart tissue lysate, Rat brain tissue lysate, Rat heart tissue lysate, rat brain tissue, PC-12.
Tissue Specificity: Low tissue specificity.
Subcellular Location: Cytoplasm, cytosol, Golgi apparatus, Endomembrane system, Mitochondrion outer membrane, Peroxisome, Membrane, clathrin-coated pit, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane.
Sequence Similarities: 100% Mouse/Rat.
Predicted band size: 82 kDa.
Target Information
A human dynamin-related protein, DRP1 contributes to mitochondrial division in mammalian cells. It plays this important role in mitochondrial fission at steady state and during apoptosis. DRP1 is required for proper cellular distribution of mitochondria, and in mutant neurons, mitochondria are largely absent from synapses, thus providing a genetic tool to assess the role of mitochondria at synapses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-terminal region; Dnm1p/Vps1p-like protein; DVLP; Dymple; dynamin 1-like; Dynamin family member proline-rich carboxyl-terminal domain less; Dynamin-1-like protein; Dynamin-like protein; dynamin-like protein 1; Dynamin-like protein 4; Dynamin-like protein IV; Dynamin-related protein 1; DYNIV 11; DYNIV-11; EC 3.6.5.5; FLJ41912; HdynIV; N-terminal region; VPS1
Gene Aliases: 6330417M19Rik; AI450666; DLP1; DNM1L; Dnml1; Dnmlp1; DRP1; DVLP; DYMPLE; EMPF; HDYNIV; python
UniProt ID: (Human) O00429, (Rat) O35303, (Mouse) Q8K1M6
Entrez Gene ID: (Human) 10059, (Rat) 114114, (Mouse) 74006
mitochondrial fission
release of cytochrome c from mitochondria
dynamin polymerization involved in mitochondrial fission
endocytosis
cellular component disassembly involved in execution phase of apoptosis
regulation of mitochondrion organization
peroxisome fission
regulation of protein oligomerization
positive regulation of apoptotic process
positive regulation of GTPase activity
mitochondrial fragmentation involved in apoptotic process
positive regulation of protein secretion
protein oligomerization
protein homotetramerization
heart contraction
membrane fusion
necroptotic process
mitochondrion morphogenesis
protein localization to mitochondrion
positive regulation of mitochondrial fission
mitochondrial membrane fission
positive regulation of release of cytochrome c from mitochondria
regulation of peroxisome organization
regulation of mitophagy
regulation of ATP metabolic process
positive regulation of intrinsic apoptotic signaling pathway
metabolic process
programmed cell death
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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