Invitrogen

Phospho-DRP1 (Ser616) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

7 Published Figures

12 References

View all (50) DNM1L antibodies

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Datasheet

Protocols

Questions & Answers

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Phospho-DRP1 (Ser616) Antibody in Western Blot (WB) — Western blot was performed using Anti-Phospho-DRP1 (Ser616) Polyclonal Antibody (Product # PA5-64821) and 58, 74 kDa bands corresponding to Phospho-DRP1 (Ser616) were observed (Fig. A). Whole cell extracts (30 µg lysate) of IMR-32 (Lane 1), SH-SY5Y (Lane 2) and Daudi (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). For Fig. B, the blot was treated with Lambda Protein Phosphatase after the protein transfer and for Fig. A, the blot was left untreated. In Fig.a, band of interest is not present in Daudi, a low expressing model for DRP1. Phospho-specificity is demonstrated by absence of band in Fig.b.

Phospho-DRP1 (Ser616) Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across the cell lines tested owing to their inherent genetic constitution. Relative expression of Phospho-DRP1 (Ser616) was observed in IMR32, SH-SY5Y in comparison to Daudi, a model that expresses low levels of DRP1 using Anti-Phospho-DRP1 (Ser616) Polyclonal Antibody (Product # PA5-64821) in Western Blot.

Product Details

PA5-64821

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human DRP1 (Phospho-Ser616)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2663284

Product Specific Information

Phospho-DRP1 (Ser616) Polyclonal Antibody detects endogenous levels of DRP1 only when phosphorylated at Ser616.

Target Information

A human dynamin-related protein, DRP1 contributes to mitochondrial division in mammalian cells. It plays this important role in mitochondrial fission at steady state and during apoptosis. DRP1 is required for proper cellular distribution of mitochondria, and in mutant neurons, mitochondria are largely absent from synapses, thus providing a genetic tool to assess the role of mitochondria at synapses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mitochondrial Fission and Mitophagy Reciprocally Orchestrate Cardiac Fibroblasts Activation.

Frontiers in cell and developmental biology

Gao QY,Zhang HF,Tao J,Chen ZT,Liu CY,Liu WH,Wu MX,Yin WY,Gao GH,Xie Y,Yang Y,Liu PM,Wang JF,Chen YX

PA5-64821 was used in Western Blot to investigate the role of mitochondrial fission in cardiac fibroblasts activation and explore the mechanisms involved in the maintenance of mitochondrial health under this condition.

Mon Sep 12 00:00:00 EDT 2022

Grb2 Induces Cardiorenal Syndrome Type 3: Roles of IL-6, Cardiomyocyte Bioenergetics, and Akt/mTOR Pathway.

Frontiers in cell and developmental biology

Wang J,Sun X,Wang X,Cui S,Liu R,Liu J,Fu B,Gong M,Wang C,Shi Y,Chen Q,Cai G,Chen X

Published figure using Phospho-DRP1 (Ser616) polyclonal antibody (Product # PA5-64821) in Western Blot

Mon Sep 05 00:00:00 EDT 2022

DRP1 Promotes BRAF^V600E-Driven Tumor Progression and Metabolic Reprogramming in Colorectal Cancer.

Frontiers in oncology

Padder RA,Bhat ZI,Ahmad Z,Singh N,Husain M

PA5-64821 was used in Western Blot to investigate how BRAFV600E driven CRC (colorectal cancer) progression is linked to mitochondrial fission/fusion dynamics and whether this window could be exploited to target CRC progression.

Thu Apr 21 00:00:00 EDT 2022

DRP1 Promotes BRAF^V600E-Driven Tumor Progression and Metabolic Reprogramming in Colorectal Cancer.

Frontiers in oncology

Padder RA,Bhat ZI,Ahmad Z,Singh N,Husain M

Thu Apr 21 00:00:00 EDT 2022

Combined Cornus Officinalis and Paeonia Lactiflora Pall Therapy Alleviates Rheumatoid Arthritis by Regulating Synovial Apoptosis via AMPK-Mediated Mitochondrial Fission.

Frontiers in pharmacology

Huang L,Hu S,Shao M,Wu X,Zhang J,Cao G

PA5-64821 was used in Western Blot to compare the therapeutic effect of Cornus officinalis and Paeonia lactiflora with that of their main active compounds, ursolic acid and paeoniflorin, respectively, and investigate the potential involvement of AMPK-mediated mitochondrial dynamics in their therapeutic action.

Wed Apr 28 00:00:00 EDT 2021

Combined Cornus Officinalis and Paeonia Lactiflora Pall Therapy Alleviates Rheumatoid Arthritis by Regulating Synovial Apoptosis via AMPK-Mediated Mitochondrial Fission.

Frontiers in pharmacology

Huang L,Hu S,Shao M,Wu X,Zhang J,Cao G

Wed Apr 28 00:00:00 EDT 2021

Low-glucose-sensitive TRPC6 dysfunction drives hypoglycemia-induced cognitive impairment in diabetes.

Clinical and translational medicine

He C,Gao P,Cui Y,Li Q,Li Y,Lu Z,Ma H,Zhao Y,Li L,Sun F,Chen X,Jia H,Liu D,Yang G,Zheng H,Zhu Z

PA5-64821 was used in Western Blotting to indicate that TRPC6 is a critical sensitive cation channel to hypoglycemia and is a promising target to prevent RH-induced cognitive impairment by properly orchestrating the mitochondrial dynamics in diabetic patients.

Thu Oct 01 00:00:00 EDT 2020

Low-glucose-sensitive TRPC6 dysfunction drives hypoglycemia-induced cognitive impairment in diabetes.

Clinical and translational medicine

He C,Gao P,Cui Y,Li Q,Li Y,Lu Z,Ma H,Zhao Y,Li L,Sun F,Chen X,Jia H,Liu D,Yang G,Zheng H,Zhu Z

Thu Oct 01 00:00:00 EDT 2020

Lung-derived HMGB1 is detrimental for vascular remodeling of metabolically imbalanced arterial macrophages.

Nature communications

Boytard L,Hadi T,Silvestro M,Qu H,Kumpfbeck A,Sleiman R,Fils KH,Alebrahim D,Boccalatte F,Kugler M,Corsica A,Gelb BE,Jacobowitz G,Miller G,Bellini C,Oakes J,Silvestre JS,Zangi L,Ramkhelawon B

PA5-64821 was used in Western Blot, Immunohistochemistry to find that inflicting acute lung injury (ALI) to mice doubles their incidence of AAA and accelerates macrophage-driven proteolytic damage of the aortic wall.

Thu Aug 27 00:00:00 EDT 2020

Lung-derived HMGB1 is detrimental for vascular remodeling of metabolically imbalanced arterial macrophages.

Nature communications

Boytard L,Hadi T,Silvestro M,Qu H,Kumpfbeck A,Sleiman R,Fils KH,Alebrahim D,Boccalatte F,Kugler M,Corsica A,Gelb BE,Jacobowitz G,Miller G,Bellini C,Oakes J,Silvestre JS,Zangi L,Ramkhelawon B

Thu Aug 27 00:00:00 EDT 2020

Lung-derived HMGB1 is detrimental for vascular remodeling of metabolically imbalanced arterial macrophages.

Nature communications

Boytard L,Hadi T,Silvestro M,Qu H,Kumpfbeck A,Sleiman R,Fils KH,Alebrahim D,Boccalatte F,Kugler M,Corsica A,Gelb BE,Jacobowitz G,Miller G,Bellini C,Oakes J,Silvestre JS,Zangi L,Ramkhelawon B

Thu Aug 27 00:00:00 EDT 2020

Adipose-Derived Mesenchymal Stem Cells Isolated from Patients with Abdominal Aortic Aneurysm Exhibit Senescence Phenomena.

Oxidative medicine and cellular longevity

Huang X,Zhang H,Liang X,Hong Y,Mao M,Han Q,He H,Tao W,Jiang G,Zhang Y,Li X

Published figure using Phospho-DRP1 (Ser616) polyclonal antibody (Product # PA5-64821) in Western Blot

Fri May 01 00:00:00 EDT 2020

FGF21 Mediates Mesenchymal Stem Cell Senescence via Regulation of Mitochondrial Dynamics.

Oxidative medicine and cellular longevity

Li X,Hong Y,He H,Jiang G,You W,Liang X,Fu Q,Han S,Lian Q,Zhang Y

PA5-64821 was used in Western Blotting to study the mechanisms of fibroblast growth factor 21 in the regulation of mesenchymal stem cell senescence.

Thu Jan 16 00:00:00 EST 2020

Haemin pre-treatment augments the cardiac protection of mesenchymal stem cells by inhibiting mitochondrial fission and improving survival.

Journal of cellular and molecular medicine

Deng R,Liu Y,He H,Zhang H,Zhao C,Cui Z,Hong Y,Li X,Lin F,Yuan D,Liang X,Zhang Y

PA5-64821 was used in Western Blotting to study regulation of mesenchymal stem cell survival by haemin.

Wed Jan 01 00:00:00 EST 2020

Vascular progenitor cell senescence in patients with Marfan syndrome.

Journal of cellular and molecular medicine

He H,Yu B,Liu Z,Ye G,You W,Hong Y,Lian Q,Zhang Y,Li X

PA5-64821 was used in Western Blotting to assess the function of Vascular progenitor cells (VPCs) isolated from patients with Marfan syndrome (MFS).

Sat Jun 01 00:00:00 EDT 2019

Cardiac Dysfunction in the Sigma 1 Receptor Knockout Mouse Associated With Impaired Mitochondrial Dynamics and Bioenergetics.

Journal of the American Heart Association

Abdullah CS,Alam S,Aishwarya R,Miriyala S,Panchatcharam M,Bhuiyan MAN,Peretik JM,Orr AW,James J,Osinska H,Robbins J,Lorenz JN,Bhuiyan MS

PA5-64821 was used in Western Blotting to investigate the physiological function of Sigmar1 in regulating cardiac hemodynamics using the Sigmar1 knockout mouse (Sigmar1-/-).

Tue Oct 16 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: C-terminal region; Dnm1p/Vps1p-like protein; DVLP; Dymple; dynamin 1-like; Dynamin family member proline-rich carboxyl-terminal domain less; Dynamin-1-like protein; Dynamin-like protein; dynamin-like protein 1; Dynamin-like protein 4; Dynamin-like protein IV; Dynamin-related protein 1; DYNIV 11; DYNIV-11; EC 3.6.5.5; FLJ41912; HdynIV; N-terminal region; VPS1

Gene Aliases: 6330417M19Rik; AI450666; DLP1; DNM1L; Dnml1; Dnmlp1; DRP1; DVLP; DYMPLE; EMPF; HDYNIV; python

UniProt ID: (Human) O00429, (Rat) O35303, (Mouse) Q8K1M6

Entrez Gene ID: (Human) 10059, (Rat) 114114, (Mouse) 74006

Molecular Function: membrane traffic protein