Western blot analysis of Phospho-Dopamine Transporter pThr53 in rat caudal lysate using a Phospho-Dopamine Transporter pThr53 polyclonal antibody (Product # PA5-35414). Results show a band at ~55kDa (glycosylated form of DAT pThr53). Immunolabeling is blocked by the phospho-peptide used as antigen (lane 2) but not by the corresponding dephospho-peptide (not shown).
|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 of rat SLC6A3 conjugated to KLH|
|Storage buffer||0.1M HEPES, pH 7.5, with 0.1mg/ml BSA, 50% glycerol, 0.15M NaCl|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody contains enough material to conduct 10 mini-Western blots. Relative mobility may vary depending on the state of glycosylation of the DAT protein. The antibody works best in lysates that have not been boiled prior to being run on an SDS-PAGE gel.
The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.