Western blot of 10 µg of rat brain synaptosomal lysate showing specific immunolabeling of the ~95 kDa dynamin phosphorylated at Ser774. Lane 1=Control. Lane 2=Peptide Block. The labeling by the antibody was blocked by the Ser774 phosphopeptide used as antigen. The corresponding dephosphopeptide did not block the immunolabeling not shown).
|Tested species reactivity||Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Sheep / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 of rat DNM1 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with bovine, canine, chicken, human, mouse and non-human primate based on 100% sequence homology. It does not cross-react with other purified substrates of cdk5 (amphiphysin and synapsin).
This antibody is specific for the ~95 kDa Dynamin protein phosphorylated at Ser774in Western blots of rat brain lysates. This antibody labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
Store at -20°C short term, 80°C long term.
Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Creation and characterization of BAC-transgenic mice with physiological overexpression of epitope-tagged RCAN1 (DSCR1).
PA1-4620 was used in western blot to develop BAC transgenic mice to study the physiological role of RCAN1 and the significance for Down syndrome
|Xing L,Salas M,Zhang H,Gittler J,Ludwig T,Lin CS,Murty VV,Silverman W,Arancio O,Tycko B||Mammalian genome : official journal of the International Mammalian Genome Society (24:30)||2013|