Western blot of 10 µg of rat brain synaptosomal lysate showing specific immunolabeling of the ~95 kDa dynamin phosphorylated at Ser778 (Lane 1). The labeling by the antibody was blocked by the Ser778 phosphopeptide used as antigen (Lane 2). The corresponding dephosphopeptide did not block the immunolabeling (not shown).
|Tested species reactivity||Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Sheep / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser778 of rat DNM1 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with bovine, canine, chicken, human and mouse based on 100% sequence homology.
This antibody is specific for the ~95 kDa Dynamin protein phosphorylated at Ser778 in Western blots of rat brain lysates.
Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Isoaspartate accumulation in mouse brain is associated with altered patterns of protein phosphorylation and acetylation, some of which are highly sex-dependent.
PA1-4621 was used in western blot to study the phosphorylation and acetylation of PIMT targets in male and female mice deleted for PIMT
|Qin Z,Kaufman RS,Khoury RN,Khoury MK,Aswad DW||PloS one (8:null)||2013|