Western blot analysis of Phospho-EEF1A2 pSer358 in mouse hippocampal lysate using a Phospho-EEF1A2 pSer358 polyclonal antibody (Product # PA5-35404). Results show a band at ~50kDa. Immunolabeling is blocked by treatment of the lysate with lambda phosphatase (P-tase) (30 minutes, 800 units/1 mg protein).
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser358 of human eEF1A2 conjugated to KLH|
|Storage buffer||0.1M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 0.1mg/ml BSA|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with chicken, non-human primate, rat, sheep and Xenopus based on 100% sequence homology.
This antibody contains enough material to conduct 10 mini-Western blots.
This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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