|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Western Blot (WB)||See 4 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human EGFR that contains tyrosine 845. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
Epidermal Growth Factor Receptor (EGFR) is a 175 kDa transmembrane glycoprotein belonging to tyrosine kinase superfamily. It acts as a receptor for epidermal growth factor (EGF) family proteins. Binding of EGFR to its ligands causes autophosphorylation of tyrosine kinase followed by activation of signal transduction pathways connected to cell proliferation and differentiation. Tyrosine 1068 present within the cytoplasmic domain of the receptor is a major autophosphorylation site that allows binding of Grb2 and activation of the Ras-Raf-ERK1 and ERK2 signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 22.214.171.124; EGFR-related peptide; Epidermal growth factor receptor; Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); Epidermal growth factor receptor precursor; epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); erb-b2 receptor tyrosine kinase 1; ERBB1; kinase EGFR; Proto-oncogene c-ErbB-1; Receptor protein-tyrosine kinase ErbB-1; Receptor tyrosine-protein kinase erbB-1; waved 2
Gene Aliases: 9030024J15Rik; AI552599; EGFR; ERBB; ErbB-1; ERBB1; Errb1; Errp; HER1; mENA; NISBD2; PIG61; wa-2; wa2; Wa5
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.