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Immunofluorescence analysis of EGFR [pY1173] was done on 70% confluent log phase HeLa cells treated with EGF (200 ng/mL for 10 min). The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with EGFR [pY1173] Rabbit polyclonal Antibody (44794G) at 2µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing cytoplasmic localization of EGFR [pY1173]. Panel e shows untreated cells. Panel f shows no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Mouse , Human , Rat|
|Published species reactivity||Mouse , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human EGFR that contains tyrosine 1173. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 7 publications below|
Epidermal Growth Factor Receptor (EGFR) is a 175 kDa transmembrane glycoprotein belonging to tyrosine kinase superfamily. It acts as a receptor for epidermal growth factor (EGF) family proteins. Binding of EGFR to its ligands causes autophosphorylation of tyrosine kinase followed by activation of signal transduction pathways connected to cell proliferation and differentiation. Tyrosine 1068 present within the cytoplasmic domain of the receptor is a major autophosphorylation site that allows binding of Grb2 and activation of the Ras-Raf-ERK1 and ERK2 signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A Monoclonal Antibody to ADAM17 Inhibits Tumor Growth by Inhibiting EGFR and Non-EGFR-Mediated Pathways.
44-794G was used in western blot to study the effect of using an ADAM17 inhibitory antibody, MEDI3622, in several EGFR-dependent tumor models.
|Rios-Doria J,Sabol D,Chesebrough J,Stewart D,Xu L,Tammali R,Cheng L,Du Q,Schifferli K,Rothstein R,Leow CC,Heidbrink-Thompson J,Jin X,Gao C,Friedman J,Wilkinson B,Damschroder M,Pierce AJ,Hollingsworth RE,Tice DA,Michelotti EF||Molecular cancer therapeutics (14:1637)||2015|
Compounds identified by virtual docking to a tetrameric EGFR extracellular domain can modulate Grb2 internalization.
44-794G was used in western blot to discuss novel EGFR-targeting therapeutics to treat cancer.
|Ramirez UD,Nikonova AS,Liu H,Pecherskaya A,Lawrence SH,Serebriiskii IG,Zhou Y,Robinson MK,Einarson MB,Golemis EA,Jaffe EK||BMC cancer (15:null)||2015|
Inhibiting heat shock protein 90 (HSP90) limits the formation of liver cysts induced by conditional deletion of Pkd1 in mice.
44-794G was used in western blot to determine the effect of STA-2842 on liver size and cystic burden in Pkd-/- mice with established polycystic liver disease.
|Smithline ZB,Nikonova AS,Hensley HH,Cai KQ,Egleston BL,Proia DA,Seeger-Nukpezah T,Golemis EA||PloS one (9:null)||2014|
Characterization of kinase inhibitors using reverse phase protein arrays.
44-794G was used in western blot to use reverse protein arrays to profile kinase inhibitors in various cellular pathways.
|Martiny-Baron G,Haasen D,D'Dorazio D,Voshol J,Fabbro D||Methods in molecular biology (Clifton, N.J.) (785:79)||2011|
Tumor growth inhibition with cetuximab and chemotherapy in non-small cell lung cancer xenografts expressing wild-type and mutated epidermal growth factor receptor.
||Steiner P,Joynes C,Bassi R,Wang S,Tonra JR,Hadari YR,Hicklin DJ||Clinical cancer research : an official journal of the American Association for Cancer Research (13:1540)||2007|
Dual inhibition of epidermal growth factor receptor and vascular endothelial growth factor receptor phosphorylation by AEE788 reduces growth and metastasis of human colon carcinoma in an orthotopic nude mouse model.
||Yokoi K,Thaker PH,Yazici S,Rebhun RR,Nam DH,He J,Kim SJ,Abbruzzese JL,Hamilton SR,Fidler IJ||Cancer research (65:3716)||2005|
Aplidin induces apoptosis in human cancer cells via glutathione depletion and sustained activation of the epidermal growth factor receptor, Src, JNK, and p38 MAPK.
||Cuadrado A,Garcia-Fernandez LF,Gonzalez L,Suarez Y,Losada A,Alcaide V,Martinez T,Fernandez-Sousa JM,Sanchez-Puelles JM,Munoz A||The Journal of biological chemistry (278:241)||2003|
ERBB1, wa-2, wa2, ERBB, Erbb, Errp, Errb1, Wa5, AI552599, HER1, mENA, ErbB-1, 9030024J15Rik, PIG61
EGFR-related peptide, Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1), avian erythroblastic leukemia viral (v-erbB) oncogene homolog, epidermal growth factor receptor, epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1), avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, waved 2, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, proto-oncogene c-ErbB-1, receptor tyrosine-protein kinase erbB-1, EC 22.214.171.124, ERBB1, Epidermal growth factor receptor precursor, Receptor protein-tyrosine kinase ErbB-1, kinase EGFR