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Immunofluorescence analysis of EGFR [pY845] was done on 70% confluent log phase HeLa cells treated with EGF (200 ng/mL for 10 min). The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with EGFR [pY845] Rabbit polyclonal Antibody (44784G) at 2µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing cytoplasmic localization. Panel e shows untreated cells. Panel f shows no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Human , Rat , Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human EGFR that contains tyrosine 845. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Epidermal Growth Factor Receptor (EGFR) is a 175 kDa transmembrane glycoprotein belonging to tyrosine kinase superfamily. It acts as a receptor for epidermal growth factor (EGF) family proteins. Binding of EGFR to its ligands causes autophosphorylation of tyrosine kinase followed by activation of signal transduction pathways connected to cell proliferation and differentiation. Tyrosine 1068 present within the cytoplasmic domain of the receptor is a major autophosphorylation site that allows binding of Grb2 and activation of the Ras-Raf-ERK1 and ERK2 signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Normalization of wound healing and stem cell marker patterns in organ-cultured human diabetic corneas by gene therapy of limbal cells.
44-784G was used in immunohistochemistry to evaluate the therapeutical effects of gene therapy of limbal cells
|Saghizadeh M,Dib CM,Brunken WJ,Ljubimov AV||Experimental eye research (129:66)||2014|
Sialyl Lewis X modification of the epidermal growth factor receptor regulates receptor function during airway epithelial wound repair.
||Allahverdian S,Wang A,Singhera GK,Wong BW,Dorscheid DR||Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology (40:607)||2010|
ERBB1, wa-2, wa2, ERBB, Erbb, Errp, Errb1, Wa5, AI552599, HER1, mENA, ErbB-1, 9030024J15Rik, PIG61
EGFR-related peptide, Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1), avian erythroblastic leukemia viral (v-erbB) oncogene homolog, epidermal growth factor receptor, epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1), avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, waved 2, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, proto-oncogene c-ErbB-1, receptor tyrosine-protein kinase erbB-1, EC 18.104.22.168, ERBB1, Epidermal growth factor receptor precursor, Receptor protein-tyrosine kinase ErbB-1, kinase EGFR