Invitrogen

Phospho-EIF4B (Ser504) Polyclonal Antibody

View all (28) eIF4B antibodies

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Datasheet

Protocols

Questions & Answers

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Phospho-EIF4B (Ser504) Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of Phospho-EIF4B (Ser504) in HeLa cells (heat shock treatment). Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114571). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.

Phospho-EIF4B (Ser504) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-EIF4B (Ser504) in mouse pancreatic tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114571) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Phospho-EIF4B (Ser504) Antibody in Western Blot (WB) — Western blot analysis of Phospho-EIF4B (Ser504) in mouse lung (left lane: treated with blocking peptide). Samples were incubated with polyclonal antibody (Product # PA5-114571).

Product Details

PA5-114571

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human eIF4B around the phosphorylation site of Ser504. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2899207

Target Information

The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.