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Phospho-EIF4B (Ser504) Antibody (PA5-114571) in ICC/IF
Immunocytochemistry analysis of Phospho-EIF4B (Ser504) in HeLa cells (heat shock treatment). Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114571). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conju... View More
Product Details
PA5-114571
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human eIF4B around the phosphorylation site of Ser504.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2899207
Product Specific Information
Antibody detects endogenous levels of eIF4B only when phosphorylated at Ser504.
Target Information
The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eIF-4B; Eukaryotic translation initiation factor 4B; hCG_2014609
Gene Aliases: 2310046H11Rik; AL024095; C85189; EIF-4B; Eif4a2; EIF4B; PRO1843
UniProt ID: (Human) P23588, (Mouse) Q8BGD9
Entrez Gene ID: (Human) 1975, (Rat) 300253, (Mouse) 75705
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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