Dot blot analysis of Phospho-EIF4EBP1 (p-4EBP1) pThr36 was performed by adsorbing 50ng of phospho-peptide (bottom) or non phospho-peptide (top) onto a nitrocellulose membrane. The membrane was blocked with 5% non-fat milk in TBST for 1 hour at room temperature and probed with a Phospho-EIF4EBP1 (p-4EBP1) pThr36 polyclonal antibody (Product # PA5-12847) at a dilution of 1:500 also for 1 hour at room temperature. The membrane was washed in TBST, probed with an anti-rabbit IgG-HRP secondary antibody, and detection was performed using a chemiluminescent substrate.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T36 of human EIF4EBP1|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Dot blot (DB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, mouse and rat based on sequence homology.
EIF4EBP1 is a member of a family of translation repressor proteins. This protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. It is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.