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|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser383 of human ELK1 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with canine, mouse, non-human primate and zebrafish based on 100% sequence homology.
This antibody is specific for ~46 kDa Elk-1 phosphorylated at Ser383 in Western blots using phospho and dephospho Elk-1 recombinant protein. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
Elk-1 is a transcription factor involved in mediating gene transcription in response to growth factors (Hill and Treisman, 1995). Elk-1 is thought to be phosphorylated by MAP kinase at Ser383 and phosphorylation at this site is essential for the transcriptional activity of Elk-1 (Li et al., 2003). Phosphorylation of Elk-1 has also been implicated in synaptic plasticity in the adult hippocampus (Thiels et al., 2002).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ELK1, member of ETS oncogene family; ETS domain protein Elk1; ETS domain-containing protein Elk-1; ETS-like gene 1; Oncogene Elk1; tyrosine kinase (ELK1) oncogene