Western blot analysis of Phospho-ETK/BMX pTyr40 using Phospho-ETK/BMX pTyr40 polyclonal antibody (Product # PA5-36787) at a dilution of 1:500. Lane 1: MCF-7 cell lysate treated with IL-1-beta (2ng/ml, 24h), Lane 2: Raw264.7 cell lysate treated with IL-1 (2ng/ml, 24h), Lane 3: H9C2 cell lysate treated with IL-1-beta (2ng/ml, 24h).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human ETK around the phosphorylation site of Tyrosine 659|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 78 kDa.
Purity is >95% by SDS-PAGE.
ETK, also known as BMX, is a member of the Bruton's tyrosine kinase (Btk) family. It is expressed in a variety of hematopoietic, epithelial and endothelial cells. Etk, like other Btk family members, contains a pleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. It participates in multiple signal transduction pathways. Phosphorylation of Tyr566 by Src kinase is required for activation of Etk in vivo. In endothelial and epithelial cells, Etk is regulated by FAK through phosphorylation at Tyr40.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.