Peptide Competition: Lysates from Jurkat cells either untreated (1, 6), treated with hydrogen peroxide (2-5), or the ionophore A23187 (7) were resolved on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 3% milk-TBST buffer
|Tested species reactivity||Chicken, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ETS1 that contains serine 251.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Ets-1 is a transcription factor involved in the regulation of genes required for extracellular matrix remodeling during metastasis of tumors. It is known to interact with the enhancer of urokinase like plasminogen activator, and promoters of stromelysin-1 and collagenase-1. It is absent from normal gastric mucosa but is expressed in 60% of gastric carcinoma and oral squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.