Lysates from Jurkat cells either untreated (1, 6), treated with hydrogen peroxide (2-5), or ionophore A23187 (7) were resolved on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 3% milk-TBST buffer for one hour at room temperature, then were incubated with the ETS1[pS 282] antibody overnight at 4%deg;C in a 1% milk-TBST buffer, following prior incubation with: no peptide (1-2, 6-7), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab′)2 anti-rabbit IgG HRP conjugate (Prod #ALI4404), and bands were detected using the Pierce SuperSignal™ method.
|Tested species reactivity||Chicken, Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ETS1 that contains serine 282.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Ets-1 is a transcription factor involved in the regulation of genes required for extracellular matrix remodeling during metastasis of tumors. It is known to interact with the enhancer of urokinase like plasminogen activator, and promoters of stromelysin-1 and collagenase-1. It is absent from normal gastric mucosa but is expressed in 60% of gastric carcinoma and oral squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Phosphorylation of ETS1 by Src family kinases prevents its recognition by the COP1 tumor suppressor.
44-1109G was used in western blot to study how phosphorylation alters COP1-binding sites of oncoproteins.
|Lu G,Zhang Q,Huang Y,Song J,Tomaino R,Ehrenberger T,Lim E,Liu W,Bronson RT,Bowden M,Brock J,Krop IE,Dillon DA,Gygi SP,Mills GB,Richardson AL,Signoretti S,Yaffe MB,Kaelin WG||Cancer cell (26:222)||2014|