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Immunofluorescence analysis of Phospho-ETS2 pThr72 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-ETS2 pThr72 Rabbit Polyclonal Antibody (441105G)at 1ug/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing Nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ETS2 that contains threonine 72.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1-5 µg|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunocytochemistry (ICC)||1-2 µg/ml|
|Immunofluorescence (IF)||1-2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Inactivation of Rb in stromal fibroblasts promotes epithelial cell invasion.
44-1105G was used in immunohistochemistry - paraffin section and western blot to study the mechanism by which inactivation of stromal fibroblast retinoblastoma protein promotes invasion of epithelial cells.
|Pickard A,Cichon AC,Barry A,Kieran D,Patel D,Hamilton P,Salto-Tellez M,James J,McCance DJ||The EMBO journal (31:3092)||2012|
|Human||Not Cited||RuvBl2 cooperates with Ets2 to transcriptionally regulate hTERT in colon cancer.||Flavin P,Redmond A,McBryan J,Cocchiglia S,Tibbitts P,Fahy-Browne P,Kay E,Treumann A,Perrem K,McIlroy M,Hill AD,Young LS||FEBS letters (585:2537)||2011|
ETS2IT1; oncogene ETS-2; protein C-ets-2; v-ets avian erythroblastosis virus E2 oncogene homolog 2; v-ets avian erythroblastosis virus E26 oncogene homolog 2; v-ets erythroblastosis virus E26 oncogene homolog 2
AU022856; Ets-2; ETS2; ETS2IT1