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|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of tyrosine 324 (G-D-Y(p)-G-H) derived from Human Ephrin B1/B2/B3 .|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for Western blot is K562 cells; suggested positive control for IHC is human brain tissue.
Eph receptors and ephrins exhibit complementary expression in many tissues during embryogenesis indicating that bidirectional activation of Eph receptors and ephrin-B proteins may occur at expression domain interfaces. Ephrin-B1 transduces outside-in signals through C-terminal protein interactions that effect integrin-mediated cell attachment and migration. The distribution of ephrin-B1 in the developing retina suggests that it influences retinal axon mapping along the dorsal-ventral axis and may be involved in intratectal development The transmembrane ligand ephrin-B2 and its receptor tyrosine kinase EphB4 are specifically expressed on arterial and venous endothelial cells, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
EFL6; EFNB2; EFNB3; ELK ligand; ELK-L; EPH related receptor transmembrane ligand ELK L3; EPH related receptor tyrosine kinase ligand 8; EPH-related receptor transmembrane ligand ELK-L3; EPH-related receptor tyrosine kinase ligand 2; EPH-related receptor tyrosine kinase ligand 5; eph-related receptor tyrosine kinase ligand 8; Ephrin B3; ephrin-B1; ephrin-B2; ephrin-B3; EPLG5; EPLG8; HTK ligand; HTKL; LERK-2; LERK-5; LERK5; LERK8; ligand of eph-related kinase 5
Cek5-L; CFND; CFNS; EFB1; EFL-3; EFL-6; EFL3; EFL6; EFNB1; EFNB2; EFNB3; ELF-2; ELF-3; Elf2; Elk-L; Elk-L3; Epl2; Epl5; Epl8; EPLG2; EPLG5; EPLG8; Htk-L; HTKL; LERK-2; LERK-5; LERK-8; LERK2; LERK5; LERK8; NLERK-1; NLERK-2; Stra1