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|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human Ephrin-B1 around the phosphorylation site of Tyrosine 317|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 38 and 45 kDa.
Purity is >95% by SDS-PAGE.
Eph receptors and ephrins exhibit complementary expression in many tissues during embryogenesis indicating that bidirectional activation of Eph receptors and ephrin-B proteins may occur at expression domain interfaces. Ephrin-B1 transduces outside-in signals through C-terminal protein interactions that effect integrin-mediated cell attachment and migration. The distribution of ephrin-B1 in the developing retina suggests that it influences retinal axon mapping along the dorsal-ventral axis and may be involved in intratectal development The transmembrane ligand ephrin-B2 and its receptor tyrosine kinase EphB4 are specifically expressed on arterial and venous endothelial cells, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CEK5 receptor ligand; CEK5-L; EFL-3; EFL3; EFNB1; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; Ephrin B1; Ephrin-B1; EphrinB1; EPLG2; LERK-2; LERK2; Stimulated by retinoic acid gene 1 protein
Cek5-L; CFND; CFNS; EFB1; EFL-3; EFL3; EFNB1; Elk-L; Epl2; EPLG2; LERK-2; LERK2; Stra1