Western blot of rat testes lysate showing specific immunolabeling of the ~46 kDa EphrinB phosphorylated at Tyr317 (Lane 1). The phosphospecificty of this labeling is shown in Lane 2 (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda phosphatase (1200 unit for 30 min) before being exposed to the Anti-Tyr317 EphrinB. The immunolabeling of the EphrinB is completely eliminated by treatment with lambda phosphatase.
|Tested species reactivity||Chicken, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr317 of chicken EPHB2 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, human, mouse, Xenopus and zebrafish based on 100% sequence homology.
This antibody is specific for the ~46 Dak EphrinB protein phosphorylated at Tyr317. Immunolabeling can be blocked by lambda-phosphatase treatment.
EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flana an and Vanderhae hen, 1998; Dufour et al , 2003; Oike et al, 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al, 2002). Recent work demonstrated that Tyr331 of EphrinB was phosphorylated in HEK293 cells after stimulation by the soluble EphB2 receptor tyrosine kinase (Kalo et al, 2001).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.