Invitrogen

Phospho-EpoR (Tyr454, Tyr456) Recombinant Polyclonal Antibody (3HCLC)

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Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (2)

Phospho-EpoR (Tyr454, Tyr456) Antibody in Western Blot (WB)

FIGURE: 1 / 2

Phospho-EpoR (Tyr454, Tyr456) Antibody (710484) in WB

Western blot analysis of EPO Receptor (pYpY454/456) was performed by loading 30 µg of HeLa, K-562 cell lysates (lane 1, 2) using Novex®NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membran... View More

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Phospho-EpoR (Tyr454, Tyr456) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of EPO Receptor (pYpY454/456) was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with EPO Receptor (pYpY454/456) Recombinant Rabbit Polyclonal Antibody (Product # 710484) at a dilution of 1:500 in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Goat anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). F-actin (Panel b: red) was stained with Alexa Fluor® 594 Phalloidin (Product # A12381). Nuclei (Panel c: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel d is a merged image showing cytoplasmic and membrane localization. Panel e shows competition with the phospho- EPO R (pYpY454/456) peptide. The images were captured using Nikon enabled with cool snap at 20X magnification.

Product Details

710484

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Polyclonal

Type

Antibody

Clone

3HCLC

Immunogen

Phosphorylation of the human EPO Receptor at the Tyr454 and Tyr456

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2532724

Product Specific Information

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Target Information

EpoR encodes the erythropoietin receptor, which belongs to the cytokine receptor family. When erythropoietin binds to this receptor, it activates the Jak2 tyrosine kinase, which in turn activates various intracellular pathways including Ras/MAP kinase, phosphatidylinositol 3-kinase, and STAT transcription factors. The stimulated erythropoietin receptor plays a role in the survival of erythroid cells. Defects in this gene may result in erythroleukemia and familial erythrocytosis. Dysregulation of this gene may also affect the growth of certain tumors. Multiple transcript variants are produced as a result of alternate splicing. Diseases associated with EPOR include Erythrocytosis, Familial, 1 and Erythroleukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EPO R; EPO-R; Erythropoietin receptor; pEpoR

Gene Aliases: EPO-R; EPOR

UniProt ID: (Human) P19235

Entrez Gene ID: (Human) 2057

Molecular Function: transmembrane signal receptor

Function(s)

signal transduction positive regulation of cytosolic calcium ion concentration brain development heart development positive regulation of cell proliferation positive regulation of neuron projection development erythropoietin-mediated signaling pathway negative regulation of neuron apoptotic process decidualization positive regulation of ERK1 and ERK2 cascade negative regulation of hydrogen peroxide-induced cell death

Process(es)

erythropoietin receptor activity protein binding identical protein binding