Invitrogen

Phospho-Ezrin/Radixin/Moesin (Thr567, Thr564, Thr558) Recombinant Rabbit Monoclonal Antibody (PSH05-73)

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Datasheet
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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers
Phospho-Ezrin/Radixin/Moesin (Thr567, Thr564, Thr558) Recombinant Rabbit Monoclonal Antibody (PSH05-73)

Product Details

MA5-56581

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

PSH05-73

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr567 of human ezrin protein.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Dry ice (international)

RRID

AB_3679607

Product Specific Information

Positive Control: HeLa starved for 3 hours then add 100 nM Calyculin A for 30 minutes cell lysate, NIH/3T3 starved for 24 hours then add 100 nM Calyculin A for 30 minutes cell lysate, C6 treated with 100 ng/mL Calyculin A for 1 hours cell lysate, mouse liver tissue, rat liver tissue.

Tissue Specificity: Low tissue specificity.

Subcellular Location: Cell membrane. Cell projection. Cytoplasm. Cytoskeleton. Membrane.

Sequence Similarities: 100% Mouse/Rat.

Predicted band size: 69 kDa.

Target Information

Moesin, a member of the talin-4.1 superfamily, is a linking protein of the submembraneous actin cytoskeleton. It is expressed in macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. It is involved in cell adhesion, migration, and organization of cell surface structures.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cytovillin; cytovillin 2; epididymis luminal protein 70; epididymis secretory protein Li 105; ESP10; Ezrin; Membrane-organizing extension spike protein; Moesin; p81; Radixin; radixin isoform; villin 2; villin 2 (ezrin); Villin-2

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Gene Aliases: AA516625; AW146364; C78546; CVIL; CVL; DFNB24; EZR; HEL-S-105; HEL70; MSN; p81; R75297; RDX; VIL2

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UniProt ID: (Human) P15311, (Mouse) P26040, (Rat) P31977, (Human) P26038, (Rat) O35763, (Mouse) P26041, (Human) P35241, (Mouse) P26043

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Entrez Gene ID: (Human) 7430, (Mouse) 22350, (Rat) 54319, (Human) 4478, (Rat) 81521, (Mouse) 17698, (Human) 5962, (Rat) 315655, (Mouse) 19684

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Function(s)
actin binding protein binding microtubule binding protein domain specific binding protein kinase A catalytic subunit binding protein kinase A regulatory subunit binding S100 protein binding poly(A) RNA binding cell adhesion molecule binding actin filament binding ATPase binding cadherin binding involved in cell-cell adhesion cytoskeletal protein binding protein complex binding structural molecule activity double-stranded RNA binding receptor binding structural constituent of cytoskeleton protein kinase binding protein binding, bridging protein homodimerization activity protein kinase A binding actin or actin-binding cytoskeletal protein
Process(es)
negative regulation of transcription from RNA polymerase II promoter intestinal D-glucose absorption sphingosine-1-phosphate signaling pathway cytoskeletal anchoring at plasma membrane leukocyte cell-cell adhesion axon guidance regulation of cell shape regulation of cell size positive regulation of gene expression protein kinase A signaling gland morphogenesis membrane to membrane docking microvillus assembly astral microtubule organization actin cytoskeleton reorganization receptor internalization regulation of microvillus length regulation of actin cytoskeleton organization cellular protein complex localization positive regulation of multicellular organism growth cortical microtubule organization establishment of epithelial cell apical/basal polarity filopodium assembly phosphatidylinositol-mediated signaling positive regulation of protein secretion negative regulation of T cell receptor signaling pathway actin filament bundle assembly establishment of centrosome localization establishment of endothelial barrier negative regulation of ERK1 and ERK2 cascade cellular response to cAMP protein localization to cell cortex establishment of protein localization to plasma membrane positive regulation of establishment of protein localization to plasma membrane negative regulation of interleukin-2 secretion regulation of NIK/NF-kappaB signaling regulation of organelle assembly terminal web assembly positive regulation of protein localization to early endosome positive regulation of cellular protein catabolic process negative regulation of p38MAPK cascade positive regulation of early endosome to late endosome transport establishment or maintenance of apical/basal cell polarity epithelial cell differentiation movement of cell or subcellular component cytoskeleton organization leukocyte migration cellular response to testosterone stimulus positive regulation of podosome assembly regulation of lymphocyte migration positive regulation of cell migration regulation of actin filament bundle assembly regulation of Rap protein signal transduction negative regulation of homotypic cell-cell adhesion negative regulation of GTPase activity regulation of GTPase activity apical protein localization establishment of protein localization negative regulation of cell size actin filament capping cellular response to thyroid hormone stimulus cell-cell adhesion regulation of ruffle assembly positive regulation of G1/S transition of mitotic cell cycle negative regulation of adherens junction organization cellular response to platelet-derived growth factor stimulus barbed-end actin filament capping
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