|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from human FAK around the phosphorylation site of Serine 843|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration is lot-specific and will vary from 0.5-0.6 mg/ml
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Regulation of FAK includes phosphorylation at multiple tyrosine and serine residues. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein metabolism and modification Protein modification Protein phosphorylation Signal transduction Intracellular signaling cascade Immunity and defense Stress response Developmental processes Apoptosis Cell structure and motility Cell structure Cell motility Cell proliferation and differentiation