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Immunofluorescent analysis of Phospho-FAK pTyr397 in A549 (top) and HeLa cells (bottom) using a Phospho-FAK pTyr397 recombinant rabbit monoclonal antibody (Product # 700255) at a dilution of 1:2000 (A549) and 1:1000 (HeLa) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Hoechst only (left), AF488 signal only (middle) and composite image (right) using an Alexa Fluor 568 Phalloidin.
|Tested species reactivity||Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 390-401 of Q05397.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-3 ug x 10^6 cells|
|Immunofluorescence (IF)||1-2 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:1000|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse, rat, primate, bovine, equine, canine, chicken, opossum, zebra finch, zebrafish, Xenopus and pufferfish based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Regulation of FAK includes phosphorylation at multiple tyrosine and serine residues. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
FAK1, FAK, p125FAK, PPP1R71, FRNK, mKIAA4203, FADK, Fadk, pp125FAK
FADK 1, focal adhesion kinase 1, focal adhesion kinase-related nonkinase, focal ashension kinase 1, pp125FAK, protein-tyrosine kinase 2, FAK-related non-kinase polypeptide, PTK2 protein tyrosine kinase 2, protein phosphatase 1 regulatory subunit 71, protein phosphatase 1, regulatory subunit 71, Focal Adhesion Kinase