Western blot detection of Phospho-FAK pSer722 in extracts of HeLa cells treated with 100 ng/mL Taxol for 24 hours. Samples were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF, followed by incubation using a Phospho-FAK pSer722 polyclonal antibody (Product # PA1-26668).
|Tested species reactivity||Human, Mouse, Rat, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide phosphopeptide corresponding to a portion of FAK containing serine 722.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26668 detects FAK (phospho S722) from human samples. PA1-26668 is expected to cross react with chicken, mouse, rat, and Xenopus laevis due to sequence homology.
PA1-26668 has been successfully used in Western blot procedures.
The PA1-26668 immunogen is a synthetic peptide phosphopeptide corresponding to a portion of FAK containing serine 722.
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.