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|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains serine 910. The sequence is conserved among multiple species including mouse, rat, chicken and frog.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. Tyrosine 397 is the autophosphorylation site of FAK, and involved in its initial activation. This phosphorylated site binds Src family SH2 domains and the p85 subunit of PI3-Kinase, and activates cell migration and invasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A multi-omics approach identifies key hubs associated with cell type-specific responses of airway epithelial cells to staphylococcal alpha-toxin.
44-596G was used in western blot to use a multi-omics strategy to elucidate the cellular programs altered by Staphylococcus aureus alpha-toxin
|Richter E,Harms M,Ventz K,Gierok P,Chilukoti RK,Hildebrandt JP,Mostertz J,Hochgräfe F||PloS one (10:null)||2015|
|Human||Not Cited||SHOC2 and CRAF mediate ERK1/2 reactivation in mutant NRAS-mediated resistance to RAF inhibitor.||Kaplan FM,Kugel CH,Dadpey N,Shao Y,Abel EV,Aplin AE||The Journal of biological chemistry (287:41797)||2012|
|Human||Not Cited||PLX4032, a selective BRAF(V600E) kinase inhibitor, activates the ERK pathway and enhances cell migration and proliferation of BRAF melanoma cells.||Halaban R,Zhang W,Bacchiocchi A,Cheng E,Parisi F,Ariyan S,Krauthammer M,McCusker JP,Kluger Y,Sznol M||Pigment cell & melanoma research (23:190)||2010|
|Mouse||Not Cited||Analyzing FAK and Pyk2 in early integrin signaling events.||Bernard-Trifilo JA,Lim ST,Hou S,Schlaepfer DD,Ilic D||Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.] (Chapter 14:null)||2006|
||Analyzing FAK and Pyk2 in early integrin signaling events.||Bernard-Trifilo JA,Lim ST,Hou S,Schlaepfer DD,Ilic D||Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.] (Chapter 14:null)||2006|
EC 18.104.22.168; FADK 1; FAK-related non-kinase polypeptide; FAK1; focal adhesion kinase isoform FAK-Del33; focal adhesion kinase-related nonkinase; protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; Protein- tyrosine kinase 2; PTK2; PTK2 protein tyrosine kinase 2
FADK; FAK; FAK1; FRNK; p125FAK; pp125FAK; PPP1R71; PTK2