HeLa cells were fixed prior to immunostaining with the Rabbit anti-FAK [pY397] monoclonal antibody. The signal was detected with an anti-rabbit FITC conjugated secondary antibody. The data shows that the antibody detected phosphorylated FAK localized at focal adhesions. Cells were counterstained with DAPI. (Cat. No. 44625G)
|Tested species reactivity||Chicken, Fruit fly, Human, Mouse, Rat, Xenopus|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 397. The sequence is conserved in mouse, rat, chicken and frog.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 11 publications below|
|Immunohistochemistry (IHC)||See 3 publications below|
|Immunocytochemistry (ICC)||See 3 publications below|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. Tyrosine 397 is the autophosphorylation site of FAK, and involved in its initial activation. This phosphorylated site binds Src family SH2 domains and the p85 subunit of PI3-Kinase, and activates cell migration and invasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
HSP70 Inhibition Limits FAK-Dependent Invasion and Enhances the Response to Melanoma Treatment with BRAF Inhibitors.
44-625G was used in immunocytochemistry and western blot to determine limits on FAK-dependent invasion and enhancement of the response to melanoma treatment with BRAF inhibitors due to HSP70 inhibition
|Budina-Kolomets A,Webster MR,Leu JI,Jennis M,Krepler C,Guerrini A,Kossenkov AV,Xu W,Karakousis G,Schuchter L,Amaravadi RK,Wu H,Yin X,Liu Q,Lu Y,Mills GB,Xu X,George DL,Weeraratna AT,Murphy ME||Cancer research (76:2720)||2016|
A novel regulatory pathway in granulosa cells, the LH/human chorionic gonadotropin-microRNA-125a-3p-Fyn pathway, is required for ovulation.
44-625G was used in western blot to determine the role of miR-125a-3p and Fyn in granulosa cells.
|Grossman H,Chuderland D,Ninio-Many L,Hasky N,Kaplan-Kraicer R,Shalgi R||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (29:3206)||2015|
A multi-omics approach identifies key hubs associated with cell type-specific responses of airway epithelial cells to staphylococcal alpha-toxin.
44-625G was used in western blot to use a multi-omics strategy to elucidate the cellular programs altered by Staphylococcus aureus alpha-toxin
|Richter E,Harms M,Ventz K,Gierok P,Chilukoti RK,Hildebrandt JP,Mostertz J,Hochgräfe F||PloS one (10:null)||2015|
MicroRNA miR-125a-3p modulates molecular pathway of motility and migration in prostate cancer cells.
44-625G was used in western blot to study miR-125a-3p in prostate cancer cells.
|Ninio-Many L,Grossman H,Levi M,Zilber S,Tsarfaty I,Shomron N,Tuvar A,Chuderland D,Stemmer SM,Ben-Aharon I,Shalgi R||Oncoscience (1:250)||2015|
Herpes simplex virus type 2 glycoprotein H interacts with integrin ¿vß3 to facilitate viral entry and calcium signaling in human genital tract epithelial cells.
44-625G was used in western blot to test if integrin alphavbeta3 signaling promotes the release of intracellular calcium and contributes to viral entry and spread
|Cheshenko N,Trepanier JB,González PA,Eugenin EA,Jacobs WR,Herold BC||Journal of virology (88:10026)||2014|
KLF8 and FAK cooperatively enrich the active MMP14 on the cell surface required for the metastatic progression of breast cancer.
44-625G was used in western blot to show that KLF8 and MMP14 signaling promotes human breast cancer invasion and metastasis.
|Lu H,Hu L,Yu L,Wang X,Urvalek AM,Li T,Shen C,Mukherjee D,Lahiri SK,Wason MS,Zhao J||Oncogene (33:2909)||2014|
Intercellular adhesion molecule-2 is involved in apical ectoplasmic specialization dynamics during spermatogenesis in the rat.
44-625G was used in western blot to assess the role of intercellular adhesion molecule-2 in the testis
|Xiao X,Cheng CY,Mruk DD||The Journal of endocrinology (216:73)||2013|
|Not Applicable||Not Cited||
Cortactin as a target for FAK in the regulation of focal adhesion dynamics.
44-625G was used in western blot to investigate the consequences of FAK and cortactin interactions
|Tomar A,Lawson C,Ghassemian M,Schlaepfer DD||PloS one (7:null)||2012|
|Rat||1:1000||Focal adhesion kinase-Tyr407 and -Tyr397 exhibit antagonistic effects on blood-testis barrier dynamics in the rat.||Lie PP,Mruk DD,Mok KW,Su L,Lee WM,Cheng CY||Proceedings of the National Academy of Sciences of the United States of America (109:12562)||2012|
|Mouse||Not Cited||Specific ß-containing integrins exert differential control on proliferation and two-dimensional collective cell migration in mammary epithelial cells.||Jeanes AI,Wang P,Moreno-Layseca P,Paul N,Cheung J,Tsang R,Akhtar N,Foster FM,Brennan K,Streuli CH||The Journal of biological chemistry (287:24103)||2012|
|Mouse||Not Cited||Analyzing FAK and Pyk2 in early integrin signaling events.||Bernard-Trifilo JA,Lim ST,Hou S,Schlaepfer DD,Ilic D||Current protocols in cell biology (Chapter 14:null)||2006|
Integrin signalling regulates the expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorin.
44-625G was used in immunohistochemistry to assess regulation of expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorinby integrin signaling
|Long K,Moss L,Laursen L,Boulter L,ffrench-Constant C||Nature communications (7:null)||2016|
Actin-bundling protein plastin 3 is a regulator of ectoplasmic specialization dynamics during spermatogenesis in the rat testis.
44-625G was used in immunohistochemistry to study the role of plastin 3 in ectoplasmic specialization dynamics during spermatogenesis in the rat testis
|Li N,Mruk DD,Wong CK,Lee WM,Han D,Cheng CY||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (29:3788)||2015|
|Not Applicable||Not Cited||
Increased expression and phosphorylation of focal adhesion kinase correlates with dysfunction in the volume-overloaded human heart.
44-625G was used in immunohistochemistry to investigate if FAK expression and phosphorylation is altered in the volume-overloaded human heart
|Lopes MM,Ribeiro GC,Tornatore TF,Clemente CF,Teixeira VP,Franchini KG||Clinical science (London, England : 1979) (113:195)||2007|
Modulation of FAK and Src adhesion signaling occurs independently of adhesion complex composition.
44-625G was used in immunocytochemistry and western blot to determine how modulation of FAK and Src adhesion signalling occurs without the need for adhesion complex composition
|Horton ER,Humphries JD,Stutchbury B,Jacquemet G,Ballestrem C,Barry ST,Humphries MJ||The Journal of cell biology (212:349)||2016|
Definition of a consensus integrin adhesome and its dynamics during adhesion complex assembly and disassembly.
44-625G was used in immunocytochemistry to characterize the dynamics of a consensus integrin adhesome during adhesion complex assembly and disassembly
|Horton ER,Byron A,Askari JA,Ng DH,Millon-Frémillon A,Robertson J,Koper EJ,Paul NR,Warwood S,Knight D,Humphries JD,Humphries MJ||Nature cell biology (17:1577)||2015|
Force engages vinculin and promotes tumor progression by enhancing PI3K activation of phosphatidylinositol (3,4,5)-triphosphate.
44-625G was used in immunocytochemistry to study the mechanism of the activation of focal adhesion protein vinculin by mechanical stress.
|Rubashkin MG,Cassereau L,Bainer R,DuFort CC,Yui Y,Ou G,Paszek MJ,Davidson MW,Chen YY,Weaver VM||Cancer research (74:4597)||2014|
Focal adhesion kinase-dependent focal adhesion recruitment of SH2 domains directs SRC into focal adhesions to regulate cell adhesion and migration.
44-625G was used in western blot to test if SRC, SHP1, and SHP2 target focal adhesions via their SH2 domains
|Wu JC,Chen YC,Kuo CT,Wenshin Yu H,Chen YQ,Chiou A,Kuo JC||Scientific reports (5:null)||2015|
Synthetic heparan sulfate oligosaccharides inhibit endothelial cell functions essential for angiogenesis.
44-625G was used in immunocytochemistry to study heparan sulfate regulated cytokine-dependent angiogenic cellular functions
|Cole CL,Hansen SU,Baráth M,Rushton G,Gardiner JM,Avizienyte E,Jayson GC||PloS one (5:null)||2010|
||Focal adhesion kinase-Tyr407 and -Tyr397 exhibit antagonistic effects on blood-testis barrier dynamics in the rat.||Lie PP,Mruk DD,Mok KW,Su L,Lee WM,Cheng CY||Proceedings of the National Academy of Sciences of the United States of America (109:12562)||2012|
||Analyzing FAK and Pyk2 in early integrin signaling events.||Bernard-Trifilo JA,Lim ST,Hou S,Schlaepfer DD,Ilic D||Current protocols in cell biology (Chapter 14:null)||2006|